低水平镉暴露对破骨细胞分化的影响

doi:10.11843/j.issn.0366-6964.2019.03.022

Abstract

Abstract:

In order to study the effect of low level cadmium (Cd) exposure on osteoclast (OC) differentiation, RAW264.7 cell (mononuclear macrophage lineage) was used as materials. In the presence of macrophage colony stimulating factor (M-CSF) and receptor-activated nuclear factor κB ligand (RANKL), treatment of different concentrations of Cd were conducted for 4 days. CCK-8 assay was used to detect changes in the viability of osteoclasts and their precursor cells. Tartrate-resistant acid phosphatase (TRAP) staining assay was used to observe the osteoclastogenesis. Laser scanning confocal microscopy was used to observe morphological changes of osteoclasts. The expression of osteoclast marker proteins and mRNA expression were detected by Western blot and qRT-PCR. The results showed that with the increase of Cd concentration, cell viability was significantly inhibited (P<0.01),which showed a concentration-effect relationship. Compared with the control group, the number and area of osteoclast production were significantly or extremely significantly decreased (P<0.05 or P<0.01). In the 2 and 5 μmol·L^-1 Cd treatment groups, the formation of osteoclasts' sealing zone was inhibited. The osteoclast-specific protein and mRNA expression levels of 2 and 5 μmol·L^-1 Cd groups were significantly or extremely significantly decreased (P<0.05 or P<0.01), which showed the dose effect. The above results indicate that low micromolar cadmium exposure can inhibit the differentiation of osteoclasts.

CLC Number:

S856.9

HE Shuangjiang, SONG Ruilong, CAO Ying, LIU Qingyang, ZHANG Chuang, LIU Zongping. Effects of Low Level Cadmium Exposure on Osteoclast Differentiation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 663-669.

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Effects of Low Level Cadmium Exposure on Osteoclast Differentiation

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